Custom rabbit antibody production antibody-antigen interaction is the key to most in vitro diagnostic assays. Antibodies with high affinities and specificities are critical for optimal performance in the assay. Pacific Immunology offers custom rabbit monoclonal antibody production using SPIN (Single Plasma Cell Interrogation) technology to screen the immunoglobulin repertoire of terminal differentiated plasma cells to generate high affinity antibodies that target unique epitopes on a given target antigen. This approach is particularly useful for the development of specialized in vitro diagnostics assays, such as immunohistochemistry and ELISA.
Custom Monoclonal Antibody Production Services: Tailored Solutions for Your Project
Compared to mice, the rabbit immune system generates antibody diversity and optimizes affinity through mechanisms that are more efficient than those of mice. For example, both mouse and rabbit B cells generate a variety of immunoglobulin V (variable), D (divergent), and J (joining) gene segments through somatic hypermutation; however, the use of these gene segments differs between species. In rabbit B cells, a significant portion of the antibody repertoire is generated through non-reciprocal somatic homologous recombination of upstream pseudo V gene loci into the recombined VDJ rearrangement. This results in an incredibly diverse immunoglobulin gene segment usage, dramatically increasing the chance of creating functional antibodies that recognize unique epitopes on a given antigen.
Furthermore, unlike hybridoma or B-cell cloning approaches, our phage display platform allows us to select rare and functional clones with unprecedented efficiency by performing unique selection strategies that are not possible in other formats. This includes a range of antibody maturation techniques, such as competition ELISA and epitope masking panning to eliminate immunodominant clones. The result is an end-to-end antibody development service that can deliver optimized humanized therapeutic antibodies to you in less than six months from initial rabbit immunization.